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dose anti cd3 cd28 beads  (Miltenyi Biotec)
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Miltenyi Biotec dose anti cd3 cd28 beads
A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, <t>CD3;</t> white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a <t>3-day-treatment</t> <t>with</t> <t>anti-CD3/CD28</t> Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.
Dose Anti Cd3 Cd28 Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+cd28/Treg+Expansion+Kit%2C+mouse/pmc13353084-91-20-27
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dose anti cd3 cd28 beads - by Bioz Stars, 2026-09
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anti cd3 cd28 antibodies  (Bio X Cell)
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Bio X Cell anti cd3 cd28 antibodies
A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, <t>CD3;</t> white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a <t>3-day-treatment</t> <t>with</t> <t>anti-CD3/CD28</t> Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.
Anti Cd3 Cd28 Antibodies, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+cd28/InVivoMAb+anti-mouse+CD28/pmc12963920-413-4-12
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anti cd3 cd28 antibodies - by Bioz Stars, 2026-09
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cts dynabeads cd3 cd28  (Miltenyi Biotec)
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Miltenyi Biotec cts dynabeads cd3 cd28
A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, <t>CD3;</t> white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a <t>3-day-treatment</t> <t>with</t> <t>anti-CD3/CD28</t> Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.
Cts Dynabeads Cd3 Cd28, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+cd28/CD3%CE%B5+Antibody%2C+anti-mouse%2C+REAfinity/bio_rxiv__64898__2026__04__09__717505-139-15-29
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cts dynabeads cd3 cd28 - by Bioz Stars, 2026-09
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soluble anti cd28  (Bio X Cell)
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Bio X Cell soluble anti cd28
A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, <t>CD3;</t> white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a <t>3-day-treatment</t> <t>with</t> <t>anti-CD3/CD28</t> Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.
Soluble Anti Cd28, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+cd28/InVivoMAb+anti-mouse+CD28/pm41923640-366-17-24
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soluble anti cd28 - by Bioz Stars, 2026-09
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anti cd28  (Bio X Cell)
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Bio X Cell anti cd28
A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, <t>CD3;</t> white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a <t>3-day-treatment</t> <t>with</t> <t>anti-CD3/CD28</t> Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.
Anti Cd28, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+cd28/InVivoMAb+anti-mouse+CD28/pm41921857-235-18-23
Average 97 stars, based on 1 article reviews
anti cd28 - by Bioz Stars, 2026-09
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anti cd28  (R&D Systems)
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R&D Systems anti cd28
A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, <t>CD3;</t> white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a <t>3-day-treatment</t> <t>with</t> <t>anti-CD3/CD28</t> Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.
Anti Cd28, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+cd28/Mouse+CD28+Antibody/pmc13065515-45-50-52
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Image Search Results


A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, CD3; white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a 3-day-treatment with anti-CD3/CD28 Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.

Journal: Cancer research

Article Title: Targeting the αvβ5 Integrin Modifies the TGF-β-Rich Tumor Microenvironment of Pancreatic Cancer

doi: 10.1158/0008-5472.CAN-25-4223

Figure Lengend Snippet: A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, CD3; white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a 3-day-treatment with anti-CD3/CD28 Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.

Article Snippet: The CD4 + T cells were cultured for 3 days with or without KPC78 cells in the presence of low dose anti-CD3/CD28 beads (x40 dilution; Cat# 130-095-925; Miltenyi Biotec), recombinant mouse TGF-β1 (5 ng/ml; Cat# 7666-MB; R&D Systems, Minneapolis, MN), and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Cat# 11271164001; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere.

Techniques: Flow Cytometry